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Cat Number:AKR3006-100ul
Size:100ul
Concentration:1mg/ml
Price:£197.00
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Shipping:£10.00

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Data Sheet
MSDS

 

KIR2DL3/CD158b2 [190IIC311], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human KIR2DL3 (19-161aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:Reacts with extracellular domain of the KIR2DL3, a member of the Killer cell immunoglobulin-liKe receptor (KIR) family. The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human NK cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unKnown ligand on target cells and inhibit cytotoxic activities.
Further Information:The antibody has been tested by ELISA, Western blot and immunofluorescent staining with flow cytometric analysis to assure specificity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Applications include flow cytometry (5-10g/1x106), ELISA (1:1,000 when tested against the immunized protein) and western blot analysis (1ug/ml). Note: this product is routinely tested on peripheral blood mononuclear cells(PBMCs) or natural Killer(NK) cells. The histogram is an example of the expected reactivity.
Synonym:Killer cell immunoglobulin-like receptor 2DL3, KIR2DL3, CD158B2, KIRCL23, NKAT2, KIR2DL3 (p58 KIR, CD158), MHC class I NK cell receptor, Natural killer-associated transcript 2, NKAT-2, NKAT2a, NKAT2b, p58 natural killer cell receptor clone CL-6, p58 NK receptor, p58.2 MHC class-I-specific NK re, Killer cell immunoglobulin-like receptor 2DL3, Killer cell immunoglobulin-like receptor 2DL3 CD158b, GL183, Killer Cell Immunoglobulin Like Receptor 2DL3, Killer inhibitory receptor CL2 3, KIR023GB, MHC Class I NK Cell Receptor, Natural Killer Associated Transcript 2, p58, p58 Natural Killer Cell Receptor Clone CL6, p58.2 MHC Class I Specific NK Receptor,
Ascension Number:P43628
NCBI EntrezGene:NP_055326
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Recombinant Proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL3 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The Recombinant Proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL3 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of KIR2DL3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human    KIR2DL3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of KIR2DL3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KIR2DL3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).