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Cat Number:ACD0802-50ul
Size:50ul
Concentration:1mg/ml
Price:£118.00
Quantity:
Shipping:£10.00

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Data Sheet
MSDS

 

CD45 [3G4], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, FACS
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human CD45 (1029-1249aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:CD45 belongs to a family of heavily glycosylated, high molecular weight transmembrane glycoproteins expressed on all cells of haematopoietic origin. Structurally heterogeneous, CD45 consists of a family of eight isoforms ranging from 180, 000 to 220, 000 MW generated by alternative splicing of three exons differentially expressed on subpopulation of lymphocytes. CD45 protein tyrosine phosphatase activity plays a central role in T-cell activation. CD45 plays an important role in signal transduction inhibition or upregulation of various immunological functions,
Further Information:The antibody has been tested by ELISA and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:protein tyrosine phosphatase, receptor type, C (PTPRC), CD45, B220, CD 45, CD45 antigen, GP180, L CA, LCA, Leukocyte common antigen, LY5, Protein tyrosine phosphatase receptor type C, Protein tyrosine phosphatase receptor type c polypeptide, PTPRC, SCID due to PTPRC deficiency, T200, T200 glycoprotein,
Ascension Number:P08575
NCBI EntrezGene:NM_002838
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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Cell lysates of Jurkat(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD45 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Cell lysates of Jurkat(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD45 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.